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BASIC concept

Researchers from London's Imperial College Centre for Synthetic Biology & Innovation

created a new DNA assembly method that is fast, accurate, modular, works exactly the same way

in every tier of hierarchical assemblies and can be completely automated.

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The BASIC DNA assembly method uses linkers to assemble DNA bioparts

stored in BASIC format in a user specified order.

 

Step 1: half-sections of linkers are ligated onto the bioparts

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Step 2: unligated linkers are removed via magnetic bead purification

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Step 3: purified parts are combined into final assembly and transformed

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What do you need to get started?

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  • Get BASIC bioparts that are ready to use and create your own BASIC parts

  • Get BASIC linker sets from Biolegio - high quality and affordable in a user friendly format

  • Get Magnetic Beads from Nimagen - Ampliclean beads are affordable and reliable

  • Get BsaI_HFv2 enzyme from NEB and T4 Ligase from Promega

  • Use the BASIC protocol for simple DNA assembly at bench scale

  • Use the BASIC app to design your constructs and workflows

  • Coming soon: Use automated BASIC on the Opentrons OT-2

Original BASIC publication

Storch, M. et al. BASIC: A New Biopart Assembly Standard for Idempotent Cloning Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology. ACS Synth Biol 4, 781–7 (2015).

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DNA assembly method review

Casini, A., Storch, M., Baldwin, G. S. & Ellis, T. Bricks and blueprints: methods and standards for DNA assembly. Nat. Rev. Mol. Cell Biol. 16, 568–76 (2015).

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