BASIC linkers are used to physically connect bioparts in the BASIC assemby method.
5 different classes of linkers are available:
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Neutral Linkers connect DNA parts with neutral sequence (45bp).
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Methylated Linkers encode BASIC prefix and suffix in methylated form, so that multiple bioparts flanked by these linkers constitute a new single BASIC biopart after the assembly.
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RBS linkers contain 1 of 3 defined 5'-UTR regions combined with 1 of 15 RBS sequences.
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Mixed RBS Linkers encode for RBS pools of 12, 24 or 36 specific RBS sequences.
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Peptide Fusion Linkers connect flanking bioparts in frame creating fusion proteins.
Each BASIC linker consists of 4 oligos, all of which are HPLC purified for highest assembly fidelity. Then 2 oligos are pre-annealed to constitute linker suffix and prefix section respectively.
Biolegio offers affordable linker sets with premixed linker oligos - ready to use,
making BASIC linker organisation easy and affordable.
